Integrating the Xpert MTB/RIF assay into a diagnostic workflow for rapid detection of Mycobacterium tuberculosis in a low-prevalence area.
نویسندگان
چکیده
The Xpert MTB/RIF assay is a rapid and fully automated real-time PCR assay. The performance of the Xpert MTB/RIF assay as a primary screening test for urgent clinical specimens was evaluated during a 2-year period. The results showed that replacing smear microscopy with the Xpert MTB/RIF assay facilitates laboratory handling and improves the sensitivity and specificity of Mycobacterium tuberculosis detection.
منابع مشابه
Reply to "performance of the Xpert MTB/RIF assay on nonrespiratory specimens and accuracy of this assay for detection of rifampin resistance in a low-prevalence setting".
In our recently published paper “Integrating the Xpert MTB/RIF Assay into a Diagnostic Workflow for Rapid Detection of Mycobacterium tuberculosis in a Low-Prevalence Area” (1), we express our concerns on the accurate reporting of RIF results by the Xpert MTB/RIF assay and its limited use for nonrespiratory samples. With interest, we read the comments of Blaich and Frei (2) on our paper, in whic...
متن کاملPerformance of the Xpert MTB/RIF assay on nonrespiratory specimens and accuracy of this assay for detection of rifampin resistance in a low-prevalence setting.
With interest, we read the paper “Integrating the Xpert MTB/ RIF Assay into a Diagnostic Workflow for Rapid Detection of Mycobacterium tuberculosis in a Low-Prevalence Area” by Deggim et al. (1). Based on the failed detection of Mycobacterium tuberculosis complex (MTB) by the Xpert assay (GeneXpert; Cepheid, Sunnyvale, CA) in 3 of 4 culture-positive nonrespiratory specimens, the authors conclud...
متن کاملRapid and Accurate Detection of Mycobacterium tuberculosis in Sputum Samples by Cepheid Xpert MTB/RIF Assay—A Clinical Validation Study
BACKGROUND A crucial impediment to global tuberculosis control is the lack of an accurate, rapid diagnostic test for detection of patients with active TB. A new, rapid diagnostic method, (Cepheid) Xpert MTB/RIF Assay, is an automated sample preparation and real-time PCR instrument, which was shown to have good potential as an alternative to current reference standard sputum microscopy and cultu...
متن کاملAn evaluation of false-positive rifampicin resistance on the Xpert MTB/RIF
BACKGROUND Mycobacterium tuberculosis (MTB) is one of the most significant causes of mortality and morbidity. Early diagnose is important especially in multiple drug resistant tuberculosis to avoid transmission. Traditional techniques requires at least one to three weeks for diagnosis of tuberculosis. Diagnostic delays with multiple drug resistant tuberculosis are associated with worse clinical...
متن کاملAdvances in tuberculosis diagnostics: the Xpert MTB/RIF assay and future prospects for a point-of-care test.
Rapid progress has been made in the development of new diagnostic assays for tuberculosis in recent years. New technologies have been developed and assessed, and are now being implemented. The Xpert MTB/RIF assay, which enables simultaneous detection of Mycobacterium tuberculosis (MTB) and rifampicin (RIF) resistance, was endorsed by WHO in December, 2010. This assay was specifically recommende...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 51 7 شماره
صفحات -
تاریخ انتشار 2013